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Am J Physiol Regul Integr Comp Physiol 285: R733-R740, 2003. First published June 12, 2003; doi:10.1152/ajpregu.00266.2003
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DEVELOPMENT AND TISSUE PLASTICITY

Exercise training differentially modifies age-associated alteration in expression of Na+-K+-ATPase subunit isoforms in rat skeletal muscles

Yuk-Chow Ng,1 Murali Nagarajan,1 Korinne N. Jew,2 Lisa C. Mace,2 and Russell L. Moore2

1Department of Pharmacology, Milton S. Hershey Medical Center, College of Medicine, The Pennsylvania State University, Hershey, Pennsylvania 17033-0850; and2Department of Integrative Physiology, University of Colorado, Boulder, Colorado 80309

Submitted 13 May 2003 ; accepted in final form 5 June 2003

The present study tests the hypothesis that endurance exercise training (ETr) reverses age-associated alterations in expression of Na+-K+-ATPase subunit isoforms in rat skeletal muscles. Expression of the isoforms was examined in 16-mo-old sedentary middle-aged, 29-mo-old sedentary senescent, and 29-mo-old treadmill exercise-trained senescent Fischer 344 x Brown Norway rats. Levels of the {alpha}1-isoform increased with age in red gastrocnemius (GR), white gastrocnemius (GW), and extensor digitorum longus (EDL) muscles, and ETr further increased its levels. Levels of the {alpha}2-isoform were unchanged in GR, had a strong trend for a decrease in GW, and decreased significantly in EDL. ETr increased expression of the {alpha}2-isoform in all three muscle groups. There was no increase in expression of the {beta}1-isoform in GR, GW, or EDL with age, whereas ETr markedly increased its levels in the muscles. There was a marked decrease with age in expression of the {beta}2-isoform in the muscle groups that was not reversed by ETr. By contrast, {beta}3-isoform levels increased with age in GR and GW, and ETr was able to reverse this increase. Na+-K+-ATPase enzyme activity was unchanged with age in GR and GW but increased in EDL. ETr increased enzyme activity in GR and GW and did not change in EDL. Myosin heavy chain isoforms in the muscle groups did not change significantly with age; ETr caused a general shift toward more oxidative fibers. Thus ETr differentially modifies age-associated alterations in expression of Na+-K+-ATPase subunit isoforms, and a mechanism(s) other than physical inactivity appears to play significant role in some of the age-associated changes.

{alpha}-subunit; {beta}-subunit; aging; gastrocnemius; extensor digitorum longus; myosin heavy chain


Address for reprint requests and other correspondence: Y.-C. Ng, Dept. of Pharmacology, College of Medicine, The Pennsylvania State University, Milton S. Hershey Medical Center, Hershey, PA 17033 (E-mail: ycn1{at}psu.edu).




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