HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/4/R741    most recent 00046.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Dasu, M. R. K. Articles by Perez-Polo, J. R. Search for Related Content PubMed PubMed Citation Articles by Dasu, M. R. K. Articles by Perez-Polo, J. R.

INFLAMMATION, CYTOKINES, AND TEMPERATURE REGULATION

IGF-I gene transfer effects on inflammatory elements present after thermal trauma

¹Shriners Hospitals for Children and Department of Surgery, and ²Department of Human Genetics and Biological Chemistry, University of Texas Medical Branch, Galveston, Texas 77550

Submitted 24 January 2003 ; accepted in final form 9 June 2003

Major thermal injury results in severe prolonged responses with three components: a hypermetabolic response, inflammatory responses, and endogenous wound-healing processes. We showed that use of liposome-mediated gene transfer of the insulin-like growth factor I (IGF-I) reduces burn-induced inflammatory responses and enhances wound healing. In the present study, we found transient increased levels of IGF-I protein in rats exposed to thermal trauma via liposomal gene transfer in an effort to define the transcriptional events that occur after IGF-I delivery at the site of injury. The beneficial effects of IGF-I gene transfer act partly via amelioration of burn-induced inflammatory responses that mediate cell death through caspase-3 activity and Bax expression. IGF-I gene transfer induces selective stimulation of activation protein-1 DNA-binding activity and activation of antiapoptotic, but not inflammatory, NF-B transcription factors. Data were consistent with our hypothesis that the beneficial effects of IGF-I gene transfer on burned rats act in part via activation protein-1 and NF-B transcriptional regulation and the concordance between the results obtained with antiapoptotic, as opposed to the proapoptotic, sequences as well as the corresponding changes in measures of cell death via Bax and caspase-3 mechanisms.

nuclear factor-B; thermal injury; activation protein-1; caspase-3; Bax

This article has been cited by other articles:

				T. H. Elsasser Insulin-like growth factor-I: a traffic control device on the road to tissue recovery Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2003; 285(4): R722 - R723. [Full Text] [PDF]