| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
WATER AND ELECTROLYTE HOMEOSTASIS
Departments of 1Physiology and 2Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229
Submitted 14 June 2004 ; accepted in final form 15 July 2004
The present study sought to determine whether water deprivation increases Fos immunoreactivity, a neuronal marker related to synaptic activation, in sympathetic-regulatory neurons of the hypothalamic paraventricular nucleus (PVN). Fluorogold (4%, 50 nl) and cholera toxin subunit B (0.25%, 20–30 nl) were microinjected into the spinal cord (T1–T3) and rostral ventrolateral medulla (RVLM), respectively. Rats were then deprived of water but not food for 48 h. Water deprivation significantly increased the number of Fos-positive nuclei throughout the dorsal, ventrolateral, and lateral parvocellular divisions of the PVN (water deprived, 215 ± 23 cells; control, 45 ± 7 cells, P < 0.01). Moreover, a significantly greater number of Fos-positive nuclei were localized in spinally projecting (11 ± 3 vs. 2 ± 1 cells, P < 0.025) and RVLM-projecting (45 ± 7 vs. 7 ± 1 cells, P < 0.025) neurons of the PVN in water-deprived vs. control rats, respectively. The majority of these double-labeled neurons was found in the ventrolateral and lateral parvocellular divisions of the ipsilateral PVN. Interestingly, a significantly greater percentage of RVLM-projecting PVN neurons were Fos positive compared with spinally projecting PVN neurons in the ventrolateral (25.8 ± 0.7 vs. 8.0 ± 1.5%, respectively, P < 0.01) and lateral (23.4 ± 2.1 vs. 5.0 ± 0.9%, respectively, P > 0.01) parvocellular divisions. In addition, we analyzed spinally projecting neurons of the RVLM and found a significantly greater percentage were Fos positive in water-deprived rats than in control rats (26 ± 3 vs. 3 ± 1%, respectively; P < 0.001). Collectively, the present findings indicate that water deprivation evokes a distinct cellular response in sympathetic-regulatory neurons of the PVN and RVLM.
hyperosmolality; hypovolemia; dehydration; sympathetic outflow; parvocellular
This article has been cited by other articles:
|
|
 |
|
  Q.-H. Chen and G. M. Toney Excitability of paraventricular nucleus neurones that project to the rostral ventrolateral medulla is regulated by small-conductance Ca2+-activated K+ channels J. Physiol., September 1, 2009; 587(17): 4235 - 4247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-G. Wei, Y. Yu, Z.-H. Zhang, and R. B. Felder Angiotensin II upregulates hypothalamic AT1 receptor expression in rats via the mitogen-activated protein kinase pathway Am J Physiol Heart Circ Physiol, May 1, 2009; 296(5): H1425 - H1433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Benedetti, R. Rorato, M. Castro, B. H. Machado, J. Antunes-Rodrigues, and L. L. K. Elias Water deprivation increases Fos expression in hypothalamic corticotropin-releasing factor neurons induced by right atrial distension in awake rats Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2008; 295(5): R1706 - R1712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Shi, M. A. Martinez, A. S. Calderon, Q. Chen, J. T. Cunningham, and G. M. Toney Intra-carotid hyperosmotic stimulation increases Fos staining in forebrain organum vasculosum laminae terminalis neurones that project to the hypothalamic paraventricular nucleus J. Physiol., November 1, 2008; 586(21): 5231 - 5245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Salter-Venzon and A. G. Watts The role of hypothalamic ingestive behavior controllers in generating dehydration anorexia: a Fos mapping study Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2008; 295(4): R1009 - R1019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-G. Wei, Y. Yu, Z.-H. Zhang, R. M. Weiss, and R. B. Felder Mitogen-Activated Protein Kinases Mediate Upregulation of Hypothalamic Angiotensin II Type 1 Receptors in Heart Failure Rats Hypertension, October 1, 2008; 52(4): 679 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-G. Wei, Y. Yu, Z.-H. Zhang, R. M. Weiss, and R. B. Felder Angiotensin II-Triggered p44/42 Mitogen-Activated Protein Kinase Mediates Sympathetic Excitation in Heart Failure Rats Hypertension, August 1, 2008; 52(2): 342 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Taylor, J. J. McCarthy, and S. D. Stocker Mice lacking the transient receptor vanilloid potential 1 channel display normal thirst responses and central Fos activation to hypernatremia Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2008; 294(4): R1285 - R1293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-H. Zhang, Y. Yu, Y.-M. Kang, S.-G. Wei, and R. B. Felder Aldosterone acts centrally to increase brain renin-angiotensin system activity and oxidative stress in normal rats Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H1067 - H1074. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Cham and E. Badoer Exposure to a hot environment can activate rostral ventrolateral medulla-projecting neurones in the hypothalamic paraventricular nucleus in conscious rats Exp Physiol, January 1, 2008; 93(1): 64 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Shi, S. D. Stocker, and G. M. Toney Organum vasculosum laminae terminalis contributes to increased sympathetic nerve activity induced by central hyperosmolality Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2007; 293(6): R2279 - R2289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Freeman and V. L. Brooks AT1 and glutamatergic receptors in paraventricular nucleus support blood pressure during water deprivation Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2007; 292(4): R1675 - R1682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Stocker, R. Meador, and J. M. Adams Neurons of the Rostral Ventrolateral Medulla Contribute to Obesity-Induced Hypertension in Rats Hypertension, March 1, 2007; 49(3): 640 - 646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. O'Donaughy, Y. Qi, and V. L. Brooks Central Action of Increased Osmolality to Support Blood Pressure in Deoxycorticosterone Acetate-Salt Rats Hypertension, October 1, 2006; 48(4): 658 - 663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Cham, R. Klein, N. C. Owens, M. Mathai, M. McKinley, and E. Badoer Activation of spinally projecting and nitrergic neurons in the PVN following heat exposure Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R91 - R101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Stocker, M. E. Wilson, C. J. Madden, U. Lone, and A. F. Sved Intravenous 6-hydroxydopamine attenuates vasopressin and oxytocin secretion stimulated by hemorrhage and hypotension but not hyperosmolality in rats Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R59 - R67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. B. Gottlieb, L. L. Ji, H. Jones, M. L. Penny, T. Fleming, and J. T. Cunningham Differential effects of water and saline intake on water deprivation-induced c-Fos staining in the rat Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1251 - R1261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. L. Brooks, Y. Qi, and T. L. O'Donaughy Increased osmolality of conscious water-deprived rats supports arterial pressure and sympathetic activity via a brain action Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1248 - R1255. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
