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DEVELOPMENTAL PHYSIOLOGY AND PREGNANCY

System and System A amino acid transporters in the feline endotheliochorial placenta

¹Academic Unit of Child Health, University of Manchester, St. Mary's Hospital, Manchester, M13 OJH; ²Waltham Centre for Pet Nutrition, Waltham on the Wolds, Melton Mowbray, Leicestershire, LE14 4RT; and ³Academic Unit of Obstetrics and Gynaecology, University of Manchester, St. Mary's Hospital, Manchester, M13 OJH, United Kingdom

Submitted 15 September 2003 ; accepted in final form 1 July 2004

There is no knowledge of the transport mechanisms by which solutes cross the cat placenta or any other endotheliochorial placenta. Here, we investigated whether the amino acid transport systems and A are present in the cat placenta using a placental fragment uptake technique. Data were compared with studies in the human placenta, in which the presence of these two transport systems has been well established. A time course of [³H]taurine (substrate for system ) and [¹⁴C]MeAIB (nonmetabolizable substrate for system A) uptake was determined in the term cat and human placental fragments in the presence and absence (choline substituted) of Na⁺, and further studies were carried out over 15 min. Taurine uptake into both cat and human placenta fragments was found to be Na⁺ and Cl^– dependent, and Na⁺-dependent taurine uptake was blocked by excess -alanine. MeAIB uptake was found to be Na⁺ dependent, and Na⁺-dependent MeAIB uptake was blocked by excess MeAIB or glycine. Western blotting and immunohistochemistry performed on cat and human placenta showed expression of TAUT and ATA2 (SNAT2), proteins associated with system and system A activity, respectively. This study therefore provides the first evidence of the presence of amino acid transport systems and A in the cat placenta.

taurine; glycine; MeAIB; syncytiotrophoblast

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