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Physiology and Pharmacology of Temperature Regulation

Reciprocal activation of HSF1 and HSF3 in brain and blood tissues: is redundancy developmentally related?

¹Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel; and ²Department of Cattle and Genetic Sciences, Institute of Animal Science, Agricultural Research Organization, Newe-Ya’ar Research Center, Ramat Yishay, Israel

Submitted 22 September 2005 ; accepted in final form 31 January 2006

Transcriptional induction of heat-shock genes in response to temperature elevation and other stresses is mediated by heat-shock transcription factors (HSFs). Avian cells express two redundant heat-shock responsive factors, HSF1 and HSF3, which differ in their activation kinetics and threshold induction temperature. Unlike the ubiquitous activation of HSF1, the DNA-binding activity of HSF3 is restricted to undifferentiated avian cells and embryonic tissues. Herein, we report a reciprocal activation of HSF1 and HSF3 in vivo. Whereas HSF1 mediates transcriptional activity only in the brain upon severe heat shock, HSF3 is exclusively activated in blood cells upon light, moderate, and severe heat shock, promoting induction of heat-shock genes. Although not activated, HSF1 is expressed in blood cell nuclei in a granular appearance, suggesting regulation of genes other than heat-shock genes. Intraspecific comparison of heat-sensitive and heat-resistant fowl strains indicates that the unique activation pattern of HSF3 in blood tissue is a general phenomenon, not related to thermal history. Taken together, HSF1 and HSF3 mediate transcriptional activity of adult tissues and differentiated cells in a nonredundant manner. Instead, an exclusive, tissue-specific activation is observed, implying that redundancy may be developmentally related. The physiological and developmental implications are discussed.

heat-shock transcription factor; heat-shock proteins; fowl; temperature regulation

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