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INFLAMMATION AND CYTOKINES

Effects of burn injury on myocardial signaling and cytokine secretion: possible role of PKC

Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas

Submitted 4 August 2006 ; accepted in final form 19 September 2006

This study examined the effects of major burn injury on the cellular distribution of several PKC isoforms in adult rat hearts and examined the hypothesis that PKC plays a regulatory role in cardiomyocyte cytokine secretion. Burn trauma was given over 40% total body surface area in Sprague-Dawley rats. An in vitro model of burn injury included addition of burn serum, 10% by volume, to primary cardiomyocyte cultures (collagen perfusion). In vivo burn injury produced redistribution of PKC, PKC, and PKC from the cytosol (soluble) to the membrane (particulate) component of the myocardium. This activation of the PKC isoforms was evident 2 h after burn injury and progressively increased over 24 h postburn. Addition of burn serum to isolated myocytes produced similar PKC isoform redistribution from the soluble to the particulate compartment, promoted myocyte Ca²⁺ and Na⁺ loading, and promoted robust myocyte secretion of inflammatory cytokines similar to that reported after in vivo burn injury. Pretreating cardiomyocytes with either calphostin or PKC inhibitory peptide, a potent inhibitor of PKC, prevented burn serum-related redistribution of the PKC isoform and prevented burn serum-related cardiomyocyte secretion of TNF-, IL-1, IL-6, and IL-10. These data suggest that the PKC isoform plays a pivotal role in myocardial inflammatory response to injury, altering cardiac function by modulating cardiomyocyte inflammatory cytokine response to injury.

cardiomyocytes; protein kinase C isoforms; protein kinase C inhibition; burn serum challenge; calphostin; protein kinase C epsilon inhibitory peptide

This article has been cited by other articles:

				J. W. Horton, J. Tan, D. J. White, and D. L. Maass Burn injury decreases myocardial Na-K-ATPase activity: role of PKC inhibition Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1684 - R1692. [Abstract] [Full Text] [PDF]