HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 292/5/R1768    most recent 00442.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Hoppe, C. C. Articles by Moritz, K. M. Search for Related Content PubMed PubMed Citation Articles by Hoppe, C. C. Articles by Moritz, K. M.

CALL FOR PAPERS
Sex Differences in Renal and Cardiovascular Function: Physiology and Pathophysiology

Effects of dietary protein restriction on nephron number in the mouse

Departments of ¹Anatomy and Cell Biology and ²Physiology, Monash University, Melbourne, Victoria, Australia

Submitted 28 June 2006 ; accepted in final form 21 January 2007

In rats, maternal protein restriction reduces nephron endowment and often leads to adult hypertension. Sex differences in these responses have been identified. The molecular and genetic bases of these phenomena can best be identified in a mouse model, but effects of maternal protein restriction on kidney development have not been examined in mice. Therefore, we determined how combined prenatal and postnatal protein restriction in mice affects organ weight, glomerular number and dimensions, and renal expression of angiotensin receptor mRNA, in both male and female offspring. C57/BL6/129sv mice received either a normal (20% wt/wt; NP) or low (9% wt/wt; LP) protein diet during gestation and postnatal life. Offspring were examined at postnatal day 30. Protein restriction retarded growth of the kidney, liver, spleen, heart, and brain. All organs except the brain weighed less in female than male offspring. Protein restriction increased normalized (to body weight) brain weight, with females having relatively heavier brains than males. The effects of protein restriction were not sex dependent, except that normalized liver weight was reduced in males but increased in females. Glomerular volume, but not number, was greater in female than in male mice. Maternal protein restriction reduced nephron endowment similarly in male and female mice. Renal expression of AT_1A receptor mRNA was approximately sixfold greater in female than male NP mice, but similar in male LP and female LP mice. We conclude that maternal protein restriction reduces nephron endowment in mice. This effect provides a basis for future studies of developmental programming in the mouse.

stereology; nephron deficit; sex; mice; kidney

This article has been cited by other articles:

				B. R. Migeon X Inactivation, Female Mosaicism, and Sex Differences in Renal Diseases J. Am. Soc. Nephrol., November 1, 2008; 19(11): 2052 - 2059. [Abstract] [Full Text] [PDF]

				R. Iliescu, R. Cazan, G. R. McLemore Jr., M. Venegas-Pont, and M. J. Ryan Renal blood flow and dynamic autoregulation in conscious mice Am J Physiol Renal Physiol, September 1, 2008; 295(3): F734 - F740. [Abstract] [Full Text] [PDF]

				R. Rexhepaj, K. M. Boini, D. Y. Huang, K. Amann, F. Artunc, K. Wang, J. J. Brosens, D. Kuhl, and F. Lang Role of maternal glucocorticoid inducible kinase SGK1 in fetal programming of blood pressure in response to prenatal diet Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R2008 - R2013. [Abstract] [Full Text] [PDF]

				A. J. Watkins, A. Wilkins, C. Cunningham, V. H. Perry, M. J. Seet, C. Osmond, J. J. Eckert, C. Torrens, F. R. A. Cagampang, J. Cleal, et al. Low protein diet fed exclusively during mouse oocyte maturation leads to behavioural and cardiovascular abnormalities in offspring J. Physiol., April 15, 2008; 586(8): 2231 - 2244. [Abstract] [Full Text] [PDF]