| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
CALL FOR PAPERS
Connexins and the Kidney
Departments of Physiology and Biophysics and Medicine, Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, California
Submitted 18 March 2007 ; accepted in final form 7 April 2008
Endothelial intracellular calcium ([Ca2+]i) plays an important role in the function of the juxtaglomerular vasculature. The present studies aimed to identify the existence and molecular elements of an endothelial calcium wave in cultured glomerular endothelial cells (GENC). GENCs on glass coverslips were loaded with Fluo-4/Fura red, and ratiometric [Ca2+]i imaging was performed using fluorescence confocal microscopy. Mechanical stimulation of a single GENC caused a nine-fold increase in [Ca2+]i, which propagated from cell to cell throughout the monolayer (7.9 ± 0.3 µm/s) in a regenerative manner (without decrement of amplitude, kinetics, and speed) over distances >400 µm. Inhibition of voltage-dependent calcium channels with nifedipine had no effect on the above parameters, but the removal of extracellular calcium reduced 
[Ca2+]i by 50%. Importantly, the gap junction uncoupler 
-glycyrrhetinic acid or knockdown of connexin 40 (Cx40) by transfecting GENCs with Cx40 short interfering RNA (siRNA) almost completely eliminated [Ca2+]i and the calcium wave. Breakdown of extracellular ATP using a scavenger cocktail (apyrase and hexokinase) or nonselective inhibition of purinergic P2 receptors with suramin, had similar blocking effects. Scraping cells off along a line eliminated physical contact between cells but did not effect calcium wave propagation. Using an ATP biosensor technique, we detected a significant elevation in extracellular ATP ( = 76 ± 2 µM) during calcium wave propagation, which was abolished by Cx40 siRNA treatment ( = 6 ± 1 µM). These studies suggest that connexin 40 hemichannels and extracellular ATP are key molecular elements of the glomerular endothelial calcium wave, which may serve important juxtaglomerular functions.
purinergic receptors; gap junction; connexin hemichannels; glomerular filtration; renin release
This article has been cited by other articles:
|
|
 |
|
  A. Sipos, S. L. Vargas, I. Toma, F. Hanner, K. Willecke, and J. Peti-Peterdi Connexin 30 Deficiency Impairs Renal Tubular ATP Release and Pressure Natriuresis J. Am. Soc. Nephrol., August 1, 2009; 20(8): 1724 - 1732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Just, L. Kurtz, C. de Wit, C. Wagner, A. Kurtz, and W. J. Arendshorst Connexin 40 Mediates the Tubuloglomerular Feedback Contribution to Renal Blood Flow Autoregulation J. Am. Soc. Nephrol., July 1, 2009; 20(7): 1577 - 1585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yao, T. Oite, and M. Kitamura Gap junctional intercellular communication in the juxtaglomerular apparatus Am J Physiol Renal Physiol, May 1, 2009; 296(5): F939 - F946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Peti-Peterdi, I. Toma, A. Sipos, and S. L. Vargas Multiphoton Imaging of Renal Regulatory Mechanisms Physiology, April 1, 2009; 24(2): 88 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. W. Inscho Mysteries of Renal Autoregulation Hypertension, February 1, 2009; 53(2): 299 - 306. [Full Text] [PDF]
|
|
|
|
 |
|
 F. Hanner, J. von Maltzahn, S. Maxeiner, I. Toma, A. Sipos, O. Kruger, K. Willecke, and J. Peti-Peterdi Connexin45 is expressed in the juxtaglomerular apparatus and is involved in the regulation of renin secretion and blood pressure Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R371 - R380. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
