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This Article Full Text Full Text (PDF) Supplemental Material All Versions of this Article: 295/3/R857    most recent 00190.2008v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Wehrwein, E. A. Articles by Kreulen, D. L. Search for Related Content PubMed PubMed Citation Articles by Wehrwein, E. A. Articles by Kreulen, D. L.

NEUROHUMORAL CONTROL OF CARDIOVASCULAR FUNCTION

Cardiac norepinephrine transporter protein expression is inversely correlated to chamber norepinephrine content

¹Department of Physiology, Michigan State University, East Lansing; ²Department of Biological Sciences, Western Michigan University, Kalamazoo; and ³Department of Chemistry and Neuroscience Program, Michigan State University, East Lansing, Michigan; and ⁴Department of Physiology and Pharmacology, Oregon Health and Sciences University, Portland, Oregon

Submitted 14 March 2008 ; accepted in final form 11 June 2008

The cardiac neuronal norepinephrine (NE) transporter (NET) in sympathetic neurons is responsible for uptake of released NE from the neuroeffector junction. The purpose of this study was to assess the chamber distribution of cardiac NET protein measured using [³H]nisoxetine binding in rat heart membranes and to correlate NE content to NET amount. In whole mounts of atria, NET was colocalized in nerve fibers with tyrosine hydroxylase (TH) immunoreactivity. NE content expressed as micrograms NE per gram tissue was lowest in the ventricles; however, NET binding was significantly higher in the left ventricle than the right ventricle and atria (P < 0.05), resulting in a significant negative correlation (r² = 0.922; P < 0.05) of NET to NE content. The neurotoxin 6-hydroxydopamine, an NET substrate, reduced NE content more in the ventricles than the atria, demonstrating functional significance of high ventricular NET binding. In summary, there is a ventricular predominance of NET binding that corresponds to a high NE reuptake capacity in the ventricles, yet negatively correlates to tissue NE content.

reuptake; 6-hydroxydopamine; stellate ganglia; noradrenaline