Sweet taste transduction involves T1R2, T1R3, gustducin and TRPM5. Because knockout (KO) mice lacking T1R3, gustducin's G subunit (Ggust) or TRPM5 exhibited greatly reduced, but not abolished responses of the chorda tympani (CT) nerve to sweet compounds, it is likely that multiple sweet transduction pathways exist. That gurmarin (Gur), a sweet taste inhibitor, inhibits some but not all mouse CT responses to sweet compounds supports the existence of multiple sweet pathways. Here, we investigated Gur-inhibition of CT responses to sweet compounds as a function of temperature in KO mice lacking T1R3, Ggust or TRPM5. In T1R3-KO mice, responses to sucrose and glucose were Gur-sensitive (GS) and displayed a temperature dependent-increase (TDI). In Ggust-KO mice, responses to sucrose and glucose were Gur-insensitive (GI) and showed a TDI. In TRPM5-KO mice, responses to glucose were GS and showed a TDI. All three KO mice exhibited no detectable responses to SC45647, and their responses to saccharin displayed neither GS nor a TDI. For all three KO mice the lingual application of pronase, another sweet response inhibitor, almost fully abolished responses to sucrose and glucose, but did not affect responses to saccharin. These results provide evidence for: 1) the existence of multiple transduction pathways underlying responses to sugars: a T1R3-independent GS pathway for sucrose and glucose, and a TRPM5-independent temperature sensitive GS pathway for glucose; 2) the requirement for Ggust in GS sweet taste responses, and; 3) the existence of a sweet-independent pathway for saccharin, in mouse taste cells on the anterior tongue.