Characterization and cardiovascular actions of endothelin-1 and endothelin-3 from the American alligator

Björn Platzack, Yuqi Wang, Dane Crossley, Valentine Lance, James W. Hicks, J. Michael Conlon
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology Published 1 February 2002 Vol. 282 no. 2, R594-R602 DOI: 10.1152/ajpregu.00733.2000

Article Figures & Data

Figures

  • Fig. 1.
    Fig. 1.

    A comparison of the amino acid sequences of the naturally occurring endothelins (ETs) identified in mammalian and nonmammalian vertebrates. The shaded residues indicate differences from the human orthologs.

  • Fig. 2.
    Fig. 2.

    Reverse-phase HPLC on a semipreparative Vydac 218TP510 (C18) column of an extract of alligator lung (A) and kidney (B), after partial purification on Sep-Pak cartridges and by gel-permeation chromatography. The fractions (1 min) containing ET-like immunoreactivity, denoted by the bar, were pooled for further purification. Dashed line shows the concentration of acetonitrile in the eluting solvent. ABS280, absorbance at 280 nm.

  • Fig. 3.
    Fig. 3.

    Purification by reverse-phase HPLC of pulmonary alligator ET-3 on a semipreparative Vydac C4 column (A), analytic Vydac C4 column (B), analytic Vydac phenyl column (C), and analytic Vydac C18 column (D). Individual peaks were collected by hand, and arrows indicate where peak collection began and ended. Alligator pulmonary ET-1 and renal ET-3 were purified under similar conditions of chromatography. ABS214, absorbance at 214 nm.

  • Fig. 4.
    Fig. 4.

    Effect on systemic arterial blood pressure (Psys; in kPa) of bolus injections of increasing doses of alligator ET-3 (10, 30, 100, and 300 pmol/kg) in a single animal.

  • Fig. 5.
    Fig. 5.

    Mean values of the absolute changes from controls of hemodynamic parameters after injection of increasing doses of alligator ET-3: systemic vascular resistance (Rsys; A), Psys (B), systemic blood flow (Qsys;C), pulmonary vascular resistance (Rpul;D), pulmonary blood pressure (Ppul;E), and pulmonary blood flow (Qpul;F). For A–C and E,n = 6; for D and F,n = 5. Error bars indicate SEs. * Statistically significant difference from control (P ≤ 0.05).

  • Fig. 6.
    Fig. 6.

    Effect on Psys (in kPa) of bolus injections of increasing doses of human/alligator ET-1 (10, 30, 100, and 300 pmol/kg) in a single animal.

  • Fig. 7.
    Fig. 7.

    Mean values of the absolute changes from controls of hemodynamic parameters after injection of increasing doses of human/alligator ET-1: Rsys (A), Psys(B), Qsys (C), Rpul(D), Ppul (E), and Qpul(F). For A–C and E,n = 8; for D and F,n = 6. Error bars indicate SEs. * Statistically significant difference from control (P ≤ 0.05).

  • Fig. 8.
    Fig. 8.

    Effect on Psys of successive bolus intra-arterial injections of human/alligator ET-1 (A),l-arginine (l-Arg; B),N ω-nitro-l-arginine methyl ester (l-NAME; C), human/alligator ET-1 afterl-NAME treatment (D), and l-Arg after l-NAME treatment (E). Data show means ± SE; n = 5. Δ Significant change in Psys after injection; * significant difference compared with injection of same drug before l-NAME treatment.

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Keywords

reptile
vasoconstrictor
vasodilatator
pulmonary
systemic
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