Table 1.

Ten potential NHE1 interactive proteins identified by AntibodyArray and their functions

ProteinFunctionReferences
SHP-2 (SH-PTP2, SH-PTP3, PTP1D, PTP2C, Syp)Widely expressed cytoplasmic tyrosine phosphatase.42, 49, 53, 61
Function in signal relay elicited by various growth factors, cytokines, antigens, and extracellular matrixes (ECM).
Clinical relevance for Noonan syndrome, diabetes, neutropenia (Kostmann syndrome)
HSP70Highly conserved molecular chaperons.5, 12, 25, 26, 43, 59
Function in facilitating protein folding and targeting to organelles or to proteolytic machines. Moreover, HSP70s are antiapoptotic and pathological settings, such as ischemia injury, inflammation, and cancer, as well as metabolic and neurodegenerative disorders.
Bin 1 (amphiphysin II)Bin-amphiphysin-Rvsp (BAR) protein family member.14, 57, 67
Primarily expressed in skeletal muscle (around T tubules) and brain (in axon initial segments and nodes of Ranvier).
Role in actin cytoskeleton organization and as a tumor suppressor that interacts with c-Myc oncoprotein.
Amphiphysin IBAR protein family member.80
Enriched in presynaptic terminals of mammalian brains.
Role in clathrin-mediated endocytosis of synaptic vesicles.
BMXNonreceptor tyrosine kinase.60
Tec kinase family member that is involved in diverse biological processes related to the control of survival and differentiation.
Implicated in Jak-STAT signaling.
Fas (CD95, APO-1, DR2)45-kDa type 1 transmembrane protein.31, 36, 45
Member of the tumor necrosis factor superfamily of receptors.
Role in apoptosis and proliferation.
CIDE-BAs DFF45-inhibitable effector, CIDE-B promotes cell death and DNA fragmentation. CD95/Fas-mediated apoptosis is enhanced by CIDEs.32
MaspinMammary serine protease inhibitor.58, 82
Role as a tumor suppressor.
CASHighly expressed in tumor cell lines and actively dividing cells.11
Role in both toxin and tumor necrosis factor-mediated cell death as well as cell proliferation.
DR4 (TRAILR1, APO-2)Member of the tumor necrosis factor superfamily of receptors.
Similar to Fas, DR4 induces apoptosis and elicits inflammatory response.37, 46
  • SHP-2, Src homology 2 domain-containing protein tyrosine phosphatase; HSP70, heat shock protein 70; BMX, bone marrow X kinase; CIDE-B, cell-death-inducing DFF45-like effector-B; CAS, cellular apoptosis susceptibility; DR4, death receptor-4.